• Genetic Resources
  • Genomic Resources
  • Recombinational Analysis
  • Trait Mapping
  • Transcriptomics
  • Functional Analysis
  • Comparative Genomics & Bioinformatics
  • Translational Research
  • Genome Manuscript
  • ICGSC
  • Home
  • Structure
  • Contact

Now the chickpea genome has been sequenced!




Coordinators :
 
oMuhammad Imtiaz, ICARDA, Syria
oBunyamin Taran, University of Saskatchewan, Canada
 
 
About :

Plant breeders are interested to have molecular markers associated with trait of interest so that they can use these markers to enhance their selection efficiency in chickpea breeding. Basically, chickpea community is interested to develop markers associated with resistance to Fusarium wilt, Ascochyta blight, Helicoverpa armigera, and tolerance to several abiotic stresses like drought, salinity, etc. In recent years, community has shown interest to map nutritional quality traits as well.

 
 
Key publications :
 
Cho S, Kumar J, Shultz JL , Anupama K, Tefera F, Muehlbauer FJ (2002) Mapping genes for double podding and other morphological traits in chickpea. Euphytica 128: 285-292

Udupa SM, Baum M (2003) Genetic dissection of pathotype-specific resistance to ascochyta blight disease in chickpea (Cicer arietinum L.) using microsatellite markers. Theor Appl Genet 106:1196-1202

Cobos MJ, Ferna´ndez MJ, Rubio J, Kharrat M, Moreno MT, Gil J, Milla´n T (2005) A linkage map of chickpea (Cicer arietinum L.) based on populations from Kabuli × Desi crosses: location of genes for resistance to Fusarium wilt race 0. Theor Appl Genet 110: 1347-1353

Kashiwagi J, Krishnamurthy L, Gaur PM, Chandra S, Upadhyaya HD (2008) Estimation of gene effects of the drought avoidance root characteristics in chickpea (C. arietinum L). Field Crops Res 105: 64-69
 
 
Updates in 2009:
 

During year 2009, interesting attempts to locate or validate resistance genes or quantitative trait loci (QTL) in chickpea genetic map have been achieved. It should be mentioned that most work has been focused either fusarium wilt or ascochyta blight. Besides, a paper related to the location of agromic traits was published.

Collaboration between a Tunisian (INRAT and Unv. Tunis) and Spanish group (UCO) permitted to map a second gene controlling fusarium wilt race 0 (Foc02/foc02) in a cluster of genes sited in LG2 (Halila et al. 2009). Authors used a RIL population derived from an intra-specific cross CA 2139 (kabuli, susceptible) × JG 62 (desi, resistant race 0) evaluated in an infected field. This time resistance was treated as a quantitative trait because in this population the allelic state for both resistance genes could not be reliably determined. The first genes conferring resistance to race 0 (Foc01/foc01) were previously mapped in LG5 (Cobos et al. 2005).

Two research groups from India () in collaboration with CSIRO (Australia) could validate the location in LG2 of resistance genes to races 1, 2 and 3 (Gowda et al 2009). These authors also worked with a RIL population derived from an intraspecific cross: JG 62 (susceptible to races 1-5) × Vijay (resistant). In this population, monogenic inheritance for resistance to the three Foc races was observed. Novel STMS markers were included in this linkage group contributing to the saturation of this important genomic area.

In relation to resistance to ascochyta blight two Indian groups (from ICARDA and Univ Raipur) studied F2 plants from a narrow cross to locate QTL contributing to resistance to an Indian isolate of ascochyta blight (AB), based on the seedling and adult plant reaction (Kottapalli et al. 2009). In this publication three QTL were reported: QTL1 mapped to LG3, and QTL2 and QTL3 both mapped to LG4. Interestingly, there are coincidences with QTL previously mapped in different genetic backgrounds.

Anbessa et al. (2009) from University of Saskatchewan (Canada) also detected QTL for ascochyta blight resistance in chickpea using four intra-specific populations. QTL were detected in groups 2, 3, 4, 6 and 8. However, QTL showing higher LOD values were placed in LG2 and LG4.

Besides, an attempt to target genomic areas conferring resistance to ascochyta blight and fusarium wilt using RGA was achieved by a Spanish group (Palomino et al. 2009) although not high significant association with any resistance gene have been found. Efforts to find candidate genes for QTL2 has been done by genome walking using SCK13603 as anchored sequence resulting that was located in a region of a putative retrotransposon (Iruela et al. 2009).

 
 
Publications in 2009:
 
Anbessa Y, Taran B, Warkentin TD, Tullu A, Vandenberg A (2009) Genetic analyses and conservation of QTL for ascochyta blight resistance in chickpea (Cicer arietinum L.). Theor Appl Genet 119: 757-765

Aryamanesh N, Nelson MN, Yan G, Clarke HJ, Siddique KHM (2010) Mapping a major gene for growth habit and QTLs for ascochyta blight resistance and flowering time in a population between chickpea and Cicer reticulatum. Euphytica doi:10.1007/s10681-009-0086-2

Chaturvedi SK, Mishra DK, Vyas P, Mishra N (2009) Breeding for cold tolerance in chickpea. Trends in Bioscience 2 (ISSN : 0974-8431)

Flowers TJ, Gaur PM, Gowda CLL, Krishnamurthy L, Samineni S, Siddique KHM, Turner NC, Vadez V, Varshney RK, Colmer TD (2009) Salt sensitivity in chickpea. Plant Cell & Environment 33: 490-509

Gowda SJ, Radhika MP, Kadoo NY, Mhase LB, Gupta VS (2009) Molecular mapping of wilt resistance genes in chickpea. Mol Breed 24: 177-183

Gurjar G, Barve M, Giri A, Gupta VS (2009) Identification of Indian pathogenic races of Fusarium oxysporum f. sp. ciceris with gene specific, ITS and random markers. Mycologia 101: 484-495

Halila I, Cobos MJ, Rubio J, Millán T, Kharrat M, Marrakchi M, Gil J (2009) Tagging and mapping a second resistance gene for Fusarium wilt race 0 in chickpea. Eur J Plant Pathol 124: 87–92

Iruela M, Pistón F, Cubero JI, Millán T, Barro F, Gil J (2009) The marker SCK13603 associated with resistance to ascochyta blight in chickpea is located in a region of a putative retrotransposon. Plant Cell Rep 28: 53–60

Kottapalli P, Gaur PM, Katiyar SK, Crouch JH, Buhariwalla HK, Pande S, Gali KK (2009) Mapping and validation of QTLs for resistance to an Indian isolate of Ascochyta blight pathogen in chickpea. Euphytica 165: 79–88

Palomino C, Fernández-Romero MD, Rubio J, Torres A, Moreno MT, Millan T (2009) Integration of new CAPS and dCAPS-RGA markers into a composite chickpea genetic map and their association with disease resistance. Theor Appl Genet 118: 671–682

Rehman AU (2009) Characterization and molecular mapping of drought tolerance in kabuli chickpea (Cicer arietinum L.). Ph.D Thesis, University of Saskatchewan, Saskatoon

Sharma M, Varshney RK, Joginedi NR, Kannan S, Hoisington D and Pande S (2009) Genetic diversity in Indian isolates of Fusarium oxysporum f. sp. ciceris, chickpea wilt pathogen. Afr J Biotech 8: 1016-1023



MANAGEMENT OFFICE:
Center of Excellence in Genomics (CEG)
ICRISAT, Patancheru 502 324, A.P., India
Tel +91 40 30713071, Fax +91 40 30713074
mailto: Varshney, Rajeev; Mahendar, T
Webmaster-ICGGC