Supplementary Table 4 Estimation of chickpea genome size based on K-mer statistics

*K	K-mer number	Peak depth	Genome size (bp)	Used bases	Used reads	Coverage (X)
17	25,095,065,055	34	738,090,148	30,914,210,575	363,696,595	41.88

^*The frequency distribution of 17-mers within the raw genomic read sequences displays 2 major peaks
(A and B). Peak A, resembles a Gaussian distribution and represents k-mers of ~0-10X coverage
which arise by chance due to sequencing errors. Peak B, corresponding to k-mers of ~20-50X coverage,
represents the majority of the genome and resembles a Poisson distribution with minor differences due to
sequencing errors, heterozygosity and repetitive DNA. The total genome size of chickpea was estimated by
obtaining the multiplication product of 17 bp and the k-mer frequency (value at y-axis) corresponding to the
coverage (value at x-axis) at Peak B (i.e. 17X Peak B frequency).